长链非编码RNA H19促进胃癌干细胞的增殖和转移  

Long non-coding RNA H19 promotes proliferation and invasion of gastric cancer stem cells

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作  者:夏荣钧 欧英富[1] 邢维山 郑琳琳[1] 于生金[1] 林黎娟[1] Xia Rongjun;Ou Yingfu;Xing Weishan;Zheng Linlin;Yu Shengjin;Lin Lijuan(Department of Molecular Medicine, Eastern Liaoning University, Dandong 118000, Liaoning Province, China;Department of Medical Oncology, Dandong First Hospital, Dandong 118000,Liaoning Province, China)

机构地区:[1]辽东学院分子医学研究室,宁省丹市宁省丹市118000 [2]丹东市第一医院肿瘤内科,辽宁省丹东市118000

出  处:《中国组织工程研究》2019年第13期2022-2027,共6页Journal of Clinical Rehabilitative Tissue Engineering Research

基  金:辽宁省自然科学基金项目(201402081).

摘  要:背景:研究发现,干扰长链非编码RNA H19的表达,可以降低胃癌细胞系MGC-803肿瘤球细胞的成瘤能力、增殖和克隆形成能力,而H19在胃癌中是否能通过影响肿瘤干细胞关键标志物Sox2的表达进而调控胃癌干细胞尚不清楚。目的:研究长链非编码RNA H19在胃癌组织及胃癌干细胞中的表达水平,并探讨其对胃癌干细胞增殖和转移的影响及作用机制。方法:qRT-PCR检测H19在38例胃癌组织、癌旁组织、胃癌干细胞CSC-G、人永生化正常胃上皮细胞GES-1中的表达;经脂质体分别转染H19 siRNA(si-H19)和阴性对照siRNA(si-NC)48 h后,软琼脂克隆形成实验、MTS实验、Transwell实验检测CSC-G细胞干性、增殖和转移能力,Western blot检测CSC-G细胞中Sox2蛋白的表达。结果与结论:①qRT-PCR结果显示H19在胃癌组织中的表达显著高于其配对的癌旁组织,且在肿瘤T分期高及有淋巴结转移的组织中高表达(P均<0.05),同时H19在CSC-G细胞中的表达显著高于GES-1细胞(P <0.05);②转染H19 siRNA后,CSC-G细胞软琼脂克隆球形成的数目显著减少,细胞增殖能力抑制、细胞侵袭能力减弱、Sox2蛋白表达减少(P均<0.05);③结果表明,H19在胃癌组织和胃癌干细胞中高表达,H19可能通过作用于干细胞相关蛋白Sox2促进胃癌干细胞的增殖和转移。BACKGROUND: Studies have shown that long non-coding RNA H19 can reduce the tumorigenic ability, proliferation and clonality of gastric cancer cell line MGC-803, and it is unclear whether H19 can regulate gastric cancer stem cells through changing the expression of Sox2, a key marker of tumor stem cells.OBJECTIVE: To study the expression levels of long non-coding RNA H19 in gastric cancer tissues and gastric cancer stem cells, and to explore its effect on the proliferation and invasion of gastric cancer stem cells and its mechanism of action.METHODS: q RT-PCR was used to detect the expression of H19 in lung cancer tissues and paracancerous tissues in 38 cases as well as in gastric cancer stem cells CSC-G and human immortalized normal gastric epithelial cells GES-1. After transfection of H19 siRNA(si-H19) and control(si-NC) for 48 hours by liposome, soft agar cloning assay was used to detect the effect of H19 expression on the stemness of CSC-G cells, MTS assay used to detect the effect of H19 downregulation on CSC-G cell proliferation, and Transwell assay used to detect the effect of H19 downregulation on CSC-G cell metastasis, and western blot assay used to detect the effect of H19 downregulation on Sox2 protein in CSC-G cells.RESULTS AND CONCLUSION:(1) The results of q RT-PCR showed that the expression of H19 in gastric cancer tissues was significantly higher than that in the matched paracancerous tissues, and it was highly expressed in tissues with high tumor T stage and lymph node metastasis(P < 0.05), while the expression of H19 in CSC-G cells was significantly higher than that in GES-1 cells(P < 0.05).(2) After transfection of H19 si RNA, the number of soft agar cloned spheres in CSC-G cells was significantly decreased, the cell proliferation and invasion abilities were inhibited and the expression of Sox2 protein was decreased(P < 0.05). In conclusion, H19 is highly expressed in gastric cancer tissues and gastric cancer stem cells. H19 may promote the proliferation and metastasis of gastric cancer stem cell

关 键 词:胃癌 肿瘤干细胞 长链非编码RNAH19 细胞增殖 细胞转移 细胞侵袭 辽宁省自然科学基金 胃肿瘤 细胞运动 组织工程 

分 类 号:R459.[医药卫生—治疗学;医药卫生—临床医学] R394.2

 

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