增塑剂 DEHP 暴露对青春期小鼠社会行为的影响  

Effects of plasticizer DEHP exposure on social behavior of adolescent mice

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作  者:李季穗 仇军辉 陈寒青 王倩倩 姚凯旺 张其臻 胡一中[1] 徐晓虹[1] LI Jisui;QIU Junhui;CHEN Hanqing;WANG Qianqian;YAO Kaiwang;ZHANG Qizhen;HU Yizhong;XU Xiaohong(College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China)

机构地区:[1]浙江师范大学化学与生命科学学院,浙江金华321004

出  处:《浙江师范大学学报:自然科学版》2019年第1期56-61,共6页Journal of Zhejiang Normal University Natural Sciences

基  金:国家自然科学基金资助项目(81472935);浙江省大学生科技创新活动计划项目(2016R404019).

摘  要:为探讨邻苯二甲酸二乙基己酯(di-ethylhexylphthalate,DEHP)对青春期小鼠社会行为的影响.以旷场行为、高架十字迷宫、强迫游泳等行为模型实验检测小鼠焦虑抑郁行为,westernblot实验检测雄激素受体(androgenreceptor,AR)、多巴胺转运体(Dopaminetransporter,DAT)、多巴胺受体(dopaminereceptor2,D2)的表达水平,激光共聚焦显微镜观测离体培养海马神经元的树突形态.研究结果表明:青春期DEHP暴露降低小鼠脑和血清雄激素水平,减少社会探究和社会交互能力,加剧焦虑抑郁状态;纹状体脑区多巴胺受体D2和雄激素受体AR水平下调;抑制神经元树突发育.总之,青春期DEHP暴露会抑制小鼠的社会行为,加剧其焦虑抑郁情绪,脑内雄激素和多巴胺递质系统活动及神经元树突形态的改变可能与此有关.It was aimed to investigate the effects of di-ethylhexyl phthalate ( DEHP ) on the social behavior of adolescent mice. The behavior was detected by open field, elevated cross maze, and forced swimming task, androgen receptor (AR) and dopamine receptor 2 (D2) were examined by Western blot analyses, and the morphology of hippocampal neurons in vitro was observed by using cultured hippocampal neuron in vitro. The results showed that adolescent DEHP exposure lowered androgen levels in the brain and serum, decreased social inquiry and social interaction, and exacerbated anxiety and depression. DEHP down-regulated the levels of D2 and AR in the striatum region of brain and decreased the density of dendritic spine of hippocampal neuron. Therefore, DEHP exposure would inhibit the social behavior and aggravate anxiety and depression of puberty mice, which might be associated with the change of sex hormone levels and the reduction of the levels of D2 and AR in the brain and the inhibition of neuronal dendritic development.

关 键 词:邻苯二甲酸二乙基己酯(DEHP) 社会行为 焦虑抑郁 雄激素受体 多巴胺受体D2 树突棘 

分 类 号:R114[医药卫生—卫生毒理学;医药卫生—公共卫生与预防医学]

 

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