一测多评法测定茵栀黄颗粒中4个黄酮类成分的含量  

Simultaneous Determination of Four Flavones in Yinzhihuang Granules by QAMS

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作  者:李艳芳 黄传亮 范建伟 刘武占 关永霞 LI Yan-fang;HUANG Chuan-liang;FAN Jian-wei;LIU Wu-zhan;GUAN Yong-xia(Lunan Hope Pharmaceutical Co., Ltd., Linyi 276006, China;State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine, Linyi 276006, China;Lunan Pharmaceutical Group Co., Ltd., Linyi 276006, China)

机构地区:[1]鲁南厚普制药有限公司,山东临沂276006 [2]中药制药共性技术国家重点实验室,山东临沂276006 [3]鲁南制药集团股份有限公司,山东临沂276006

出  处:《食品与药品》2019年第1期32-36,共5页Food and Drug

摘  要:目的建立以黄芩苷为内参物,同时测定茵栀黄颗粒中野黄芩苷、汉黄芩苷、黄芩素和汉黄芩素含量的一测多评法(QAMS)。方法采用DiamonsiC18色谱柱(4.6mm×250mm,5μm),以乙腈-0.1%磷酸为流动相进行梯度洗脱,流速1.0ml/min,检测波长274nm,柱温30℃;以黄芩苷为内参物,建立与其他4个黄酮类成分的相对校正因子;分别采用外标法和一测多评法测定4个黄酮类成分的含量,比较两种计算结果的差异。结果在一定线性范围内,黄芩苷与野黄芩苷、汉黄芩苷、黄芩素和汉黄芩素的相对校正因子分别为1.721,0.821,0.601,0.567。建立的校正因子重现性良好;一测多评法计算结果与外标法测得结果无显著差异。结论建立的一测多评法可作为茵栀黄颗粒中4个黄酮类成分的测定方法,为茵栀黄颗粒的质量控制提供参考。Objective To develop a QAMS method for simultaneous multi-component determination of scutellarin, wogonoside, baicalein and wogonin in Yinzhihuang Granules with baicalin as the internal reference. Mothods Diamonsi C18 column (4.6 mm×250 mm, 5 μm) was adopted. The gradient elution was performed using acetonitrile and 0.1 % phosphoric acidmobile as mobile phase. The flow rate was 1.0 ml/min, the detection wavelength was set at 274 nm, and the column temperature was 30 ℃. Baicalin was selected as the internal standard to establish the relative correction factors (RCF) of other flavones. The contents of four flavones were determined by both external standard method and QAMS. The validity of the QAMS method was evaluated by comparison of the quantitative results of both methods. Results Under the defined ranges of determination, RCFs of scutellarin, wogonoside, baicalein and wogonin with reference to baicalin, were 1.721, 0.821, 0.601 and 0.567, respectively, and the repeatability was good under different experimental conditions. There were no obvious difference between the quantitative results of the two methods. Conclusion QAMS can be used to determine four flavones in Yinzhihuang Granules, and provide a useful approach for the qualtity control of Yinzhihuang Granules.

关 键 词:一测多评法 校正因子 茵栀黄颗粒 黄酮类成分 HPLC 

分 类 号:R917[医药卫生—药物分析学;医药卫生—药学]

 

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