番茄NBS-LRR抗根结线虫基因同源序列的克隆与分析  

Cloning and analysis of root knot nematode resistance gene of NBS-LRR analogs from tomato

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作  者:陆秀红[1] 张雨[2] 秦舒婷 黄金玲[1] 张禹[1] 刘志明[1] LU Xiuhong;ZHANG Yu;QIN Shuting;HUANG Jinling;ZHANG Yu;LIU Zhiming(Institute of Plant Protection,Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests,Nanning 530007,China;College of Arts and Sciences,Guangxi University,Nanning 530005,China)

机构地区:[1]广西农业科学院植物保护研究所/广西作物病虫害生物学重点实验室,南宁530007 [2]广西大学行建文理学院,南宁530005

出  处:《华中农业大学学报》2019年第1期67-72,共6页Journal of Huazhong Agricultural University

基  金:国家自然科学基金项目(31460465,31860492);广西科技重大专项(桂AA17204041);广西农业科学院科技发展基金项目(2015JZ20).

摘  要:根据已知抗病基因的NBS-LRR保守结构域设计简并引物,从抗南方根结线虫病的番茄材料cDNA中克隆抗病基因同源序列(resistance gene analogs,RGAs),并通过qRT-PCR技术检测RGAs与南方根结线虫侵染的相关性。序列分析发现,2条序列具有NBS-LRR保守结构域,分别命名为F5-8和F5-20;保守区域氨基酸比对结果显示,F5-8(GenBank登录号为MG860907)和F5-20(GenBank登录号为MG860908)与已知抗病基因保守区域具有很高的相似性,其中F5-8与Mi-1相应区域氨基酸相似性为94%,F5-20与Hero基因相应区域氨基酸相似性为96%。qRT-PCR结果发现,F5-8和F5-20在接种南方根结线虫2龄幼虫24 h后其表达量均发生了变化。初步推测F5-8和F5-20为南方根线线虫侵染相关基因同源序列。Degenerated primers were designed according to the conserved regions of NBS-LRR domain from known plant resistance genes.Then these primers were used for amplification of the NBS-LRR analogs from Lycopersicon esculentum.Sequence analysis showed that 5 fragments contained the resistance gene analogs,two of which have the ORF region and conserved NBS-LRR domain.F5-8(MG860907)shared 94%identity with the resistance genes of Mi-1,and F5-20(MG860908)shared 96%identity with the resistance genes of Hero.The quantitative real-time PCR results showed that expression of F5-8 and F5-20 was changed after Meloidogyne incognita infection,suggesting that F5-8 and F5-20 might be related with nematode infection.

关 键 词:番茄 南方根结线虫 抗病基因同源序列 NBS-LRR 

分 类 号:S432.4[农业科学—植物病理学;农业科学—农业昆虫与害虫防治;农业科学—植物保护]

 

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