云南甘蔗白条病的多基因联合鉴定  

Identification of sugarcane leaf scald based on multiple gene sequences in Yunnan Province

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作  者:张荣跃[1] 李文凤[1] 王晓燕[1] 单红丽[1] 仓晓燕[1] 李婕 罗志明[1] 尹炯[1] 黄应昆 ZHANG Rongyue;LI Wenfeng;WANG Xiaoyan;SHAN Hongli;CANG Xiaoyan;LI Jie;LUO Zhiming;YIN Jiong;HUANG Yingkun(Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan 661699, China)

机构地区:[1]云南省农业科学院甘蔗研究所,云南省甘蔗遗传改良重点实验室,开远661699

出  处:《植物保护》2019年第1期181-185,215共6页Plant Protection

基  金:国家现代农业糖料产业技术体系建设专项(CARS-170303);云岭产业技术领军人才培养项目(2018LJRC56);云南省现代农业产业技术体系建设专项(YNGZTX-4-92).

摘  要:甘蔗白条病是由白条黄单胞菌 Xanthomonas albilineans (Ashby) Dowson引起的甘蔗重要病害。2017年在云南保山、孟定和金平蔗区的甘蔗上发现疑似白条病的病害。从病株蔗茎中分离得到圆形,凸面,光滑,有光泽,黄色的细菌菌落。经柯赫氏法则验证,分离得到的细菌为该病害的病原菌。使用16S rRNA基因、 gyrB 基因和 rpoD 基因对分离得到的病原菌进行了分子鉴定,BLAST和系统发育分析表明本研究所获得的病原菌的3个基因的核苷酸序列与白条黄单胞菌GPE PC73菌株(GenBank登录号: FP565176)对应基因的核苷酸序列一致性均为100%,在系统发育树中处于同一分支。根据田间症状诊断、柯赫氏法则验证及分子鉴定结果,确认云南保山、孟定和金平发生的甘蔗病害为 X. albilineans 引起的甘蔗白条病。Leaf scald caused by Xanthomonas albilineans (Ashby) Dowson is an important disease of sugarcane. In 2017, suspected sugarcane leaf scald was found in Baoshan, Mengding and Jinping of Yunnan Province. Circular, convex, smooth, shiny, yellow pigmented bacterial colonies were isolated from symptomatic stalks. The result of Koch’s postulates showed that the isolated bacteria were the causal agent of the disease. Molecular identification of pathogenic bacteria was carried out based on 16S rRNA gene, gyrB gene and rpoD gene. BLAST analysis showed that the amplified nucleotide sequences of three genes shared 100% sequence identity with that of corresponding genes of X. albilineans strain GPE PC73 (accession no. FP565176). Phylogenetic analysis showed that all isolates in this study and X. albilineans were clustered together into one branch. Base on the result of symptom diagnosis in the field, validation of Koch’s postulates and molecular identification, the sugarcane disease occurred in Baoshan, Mengding and Jinping was confirmed to be leaf scald caused by X. albilineans .

关 键 词:甘蔗白条病 白条黄单胞菌 系统发育分析 云南 

分 类 号:S432.42[农业科学—植物病理学;农业科学—农业昆虫与害虫防治;农业科学—植物保护]

 

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