不同焦油释放量卷烟诱导TK基因突变能力的比较研究  

Mutagenicity of cigarette samples with different tar releases in the TK gene mutation assay

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作  者:尚平平[1] 华辰凤 谢复炜[1] 李翔[1] SHANG Pingping;HUA Chenfeng;XIE Fuwei;LI Xiang(Zhengzhou Tobacco Research Institute,China Tobacco Corporation,Zhengzhou 450001,Henan,China)

机构地区:[1]中国烟草总公司郑州烟草研究院,河南郑州450001

出  处:《癌变.畸变.突变》2019年第1期49-52,共4页Carcinogenesis,Teratogenesis and Mutagenesis

基  金:郑州烟草研究院院长科技发展基金(322016CA0240).

摘  要:目的:研究卷烟烟气冷凝物对TK基因的致突变作用,并比较不同焦油释放量卷烟样品的TK基因致突变能力。方法:以盒标焦油为5、8和11 mg的市售卷烟(分别为1、2和3号样品)烟气冷凝物为受试物,分别以20、40、60、80、100、150μg/mL的卷烟烟气冷凝物对小鼠淋巴瘤细胞L5178Ytk^+/-3.7.2C进行染毒处理,以15μL/mL的二甲基亚砜为溶剂对照,3μg/mL的环磷酰胺为阳性对照,处理3 h,细胞洗涤重新接种培养2 d,加入三氟胸苷继续培养12 d后,计数有突变集落生长的孔数,计算各样品剂量组的三氟胸苷抗性突变频率,并比较不同样品的致突变强度。结果:1号样品在剂量为150μg/mL时、2号样品和3号样品在剂量为100μg/mL时,小鼠淋巴瘤细胞三氟胸苷抗性突变频率是溶剂对照组3倍以上,并且随着卷烟烟气冷凝物剂量的增加,三氟胸苷抗性突变频率呈上升趋势,呈现明显的剂量-反应关系,3种卷烟样品的TK基因致突变强度分别为0.37、0.36和0.38。结论:3种卷烟样品的烟气冷凝物均对小鼠淋巴瘤细胞表现出TK基因致突变作用,比较3种样品的致突变强度发现,卷烟烟气的致突变强度与焦油释放量无明显相关关系。OBJECTIVE:To study the mutagenicity of cigarette smoke condensates(CSC)in the TK gene mutation assay and to compare the mutagenicity of TK gene in cigarette samples with different tar release.METHODS:3 commercial cigarettes of different tar releases,5 mg(1#),8 mg(2#)and 11 mg(3#),were tested.The mouse lymphoma cell line L5178Y tk^+/-3.7.2C was treated with 20μg/mL,40μg/mL,60μg/mL,80μg/mL,100μg/mL and 150μg/mL CSC from the 3 cigarette samples.15μL/mL DMSO treatment was used as the solvent control,and 3μg/mL cyclophosphamide treatment was used as the positive control.The test protocol includeds the 4-h treatment,2-day expression and 12-day cloning phases.Then the mutation frequency of trifluorothymidine resistance(TMF)of each dose group was calculated.Finally,the mutagenic potency of different cigarette samples was compared.RESULTS:At the dose of 150μg/mL of 1^#,100μg/mL of 2^#and 3^#,the TMF were more than 3 times higher than that in the solvent control group.In addition,the TMF increased with increase of the CSC doses.The mutagenic potency of the CSCs for 3 cigarette samples were 0.37,0.36 and 0.38,respectively.CONCLUSION:All CSCs induced dose-dependent increases of mutagenicity in the mouse lymphoma assay.Comparing the mutagenicity of the 3 cigarette samples,there was no significant correlation between TK gene mutagenic potency and tar release in cigarette smoke.

关 键 词:卷烟烟气冷凝物 焦油 TK基因突变试验 致突变强度 

分 类 号:R994.6[医药卫生—毒理学;医药卫生—药学]

 

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