甲醛对HepG2细胞游离胆固醇代谢的影响  

Effects of formaldehyde on free cholesterol metabolism in HepG2 cells

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作  者:姜雪霞 王盼[1] 刘艳飞[1] 张艳[1] 白剑英[1] JIANG Xuexia;WANG Pan;LIU Yanfei;ZHANG Yan;BAI Jianying(Department of Environment Health,School of Public Health,Shanxi Medical University,Taiyuan 030001,Shanxi,China)

机构地区:[1]山西医科大学公共卫生学院环卫教研室,山西太原030001

出  处:《癌变.畸变.突变》2019年第1期35-40,共6页Carcinogenesis,Teratogenesis and Mutagenesis

基  金:山西省留学回国人员科研资助项目(2014-034);山西省自然科学基金项目(201701D121140).

摘  要:目的:研究甲醛(FA)对人肝癌细胞株HepG2细胞游离胆固醇(FC)含量的影响及其作用机制。方法:分别采用0.004、0.02、0.1 mmol/L FA处理人肝癌细胞株HepG2细胞24和48 h,以完全培养基为阴性对照,过氧化物酶法测定HepG2细胞内FC含量;采用Western blot法检测HepG2细胞固醇调节元件结合蛋白-2(SREBP-2)、羟甲基戊二酸单酰辅酶A还原酶(HMGCR)、胆固醇酰基转移酶(ACAT)和低密度脂蛋白受体(LDLR)的蛋白表达水平。结果:与阴性对照组相比,各浓度的FA染毒24 h或0.02和0.1 mmol/L FA染毒48 h后,HepG2细胞内FC含量均明显增加(P均<0.05);FA染毒24和48 h后细胞内HMGCR蛋白表达水平均明显增加(P<0.05);FA染毒24 h后,ACAT和LDLR表达水平均明显降低(P均<0.05);FA染毒48 h,LDLR蛋白表达水平明显降低(P<0.05),ACAT蛋白表达水平在0.02和0.1 mmol/L FA组明显降低(P<0.05)。结论:甲醛染毒可使HepG2细胞内FC含量增加,可能与胆固醇的从头合成增加和胆固醇酯化水平降低有关。OBJECTIVE:To explore effects of formaldehyde(FA)on intra-hepatocellular free cholesterol content in HepG2 cells.METHODS:HepG2 cells were treated with formaldehyde at 0.004,0.02 and 0.1 mmol/L concentrations for 24 and 48 hours,respectively.The content of intra-hepatocellular free cholesterol was detected by using free the cholesterol GPO-POD assay kit.Western blot was used to detect protein expression of the sterol regulatory element-binding protein(SREBP)-2,hydroxy mothylglutaryl coenzyme A reductase(HMGCR),acyl coenzyme A-cholesterol acyltransferase(ACAT)and low-density lipoprotein receptor(LDLR).RESULTS:Compared with negative control,the contents of intra-hepatocellular FC were increased significantly after treatments with FA for 24 h and 0.02 and 0.1 mmol/L for 48 h(P<0.05).HMGCR expression levels were significantly increased after exposure to formaldehyde for 24 and 48 h(P<0.05).After treatment with FA for 24 h,expression levels of ACAT and LDLR were significantly down-regulated(P<0.05).After 48 h,expression levels of LDLR were decreased significantly,whereas the levels of ACAT were decreased significantly after treatment with 0.02 and 0.1 mmol/L FA(P<0.05).CONCLUSION:FA exposure increased intra-hepatocellular free cholesterol contents in HepG2 cells.The increase could be caused by elevated de novo synthesis of cholesterol and decreased cholesterol esterification.

关 键 词:甲醛 固醇调节元件结合蛋白2 羟甲基戊二酸单酰辅酶A还原酶 胆固醇酰基转移酶 低密度脂蛋白受体 

分 类 号:R114[医药卫生—卫生毒理学;医药卫生—公共卫生与预防医学]

 

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