车前草中大车前苷的含量测定及提取工艺优选  

Determination and optimization of extraction process of the content of plantamajoside in plantain

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作  者:吕昂[1] 范新[1] 苏倩 俞文英[3] 余陈欢[3] LU Ang;FAN Xin;SU Qian;YU Wenying;YU Chenhuan(Pharmaceutical Department,Hangzhou First People′s Hospital,Hangzhou 310006,China;Pharmaceutical Department,Hangzhou Obstetrics and Gynecology Hospital,Hangzhou 310008,China;Zhejiang Academy of Medical Sciences,Hangzhou 310013,China)

机构地区:[1]杭州市第一人民医院药学部,浙江杭州310006 [2]杭州市妇产科医院药学部,浙江杭州310008 [3]浙江省医学科学院,浙江杭州310013

出  处:《药学实践杂志》2019年第1期77-79,90共4页The Journal of Pharmaceutical Practice

基  金:浙江省中医药科技计划项目(2013ZA103).

摘  要:目的建立车前草中大车前苷的含量测定方法,并优选其提取工艺。方法采用HPLC法测定大车前苷的含量,并通过正交设计考察乙醇浓度、用量及提取时间对车前草中大车前苷提取工艺的影响。结果大车前苷在12.51~125.10μg/ml浓度范围内,线性关系良好(r=0.999 6),平均加样回收率为98.57%,RSD为1.45%;最佳提取工艺为10倍量60%乙醇提取2次,每次1h。结论本研究建立的含量测定方法简便、准确、重复性好;优选的提取工艺稳定可行。Objective To establish a method for determination and optimize the extraction process of the content of plantamajoside in plantain.Methods Plantamajoside content was determined by HPLC.The effects of ethanol concentration,ethanol amount and extraction time on the extraction of plantamajoside from plantain were studied by orthogonal design.Results The calibration curve was linear(r=0.999 6)over the range of 12.52-125.10μg/ml.The average recovery was 98.57%(RSD=1.45%).The optimum extraction process was as follows:60%ethanol,10 times volumes,extracted 2 times,1 h each time.Conclusion The established method was simple,accurate and reproducible for determination of the content of plantamajoside in plantain.The optimal extraction process was stable and feasible.

关 键 词:车前草 大车前苷 高效液相色谱 提取工艺 正交试验 

分 类 号:R917[医药卫生—药物分析学;医药卫生—药学]

 

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