镧对仔鼠皮质神经细胞BAX表达的影响  

Effect of lanthanum on BAX expression in cortical nerve cells of offspring rats

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作  者:吴天威 郭颍 王金婷 马兰 黄雅莉 郑琳琳[1] WU Tian-wei;GUO Ying;WANG Jin-ting;MA Lan;HUANG Ya-li;ZHENG Lin-lin(School of Medicine,Eastern Liaoning University,Dandong 118003,China)

机构地区:[1]辽东学院医学院,辽宁丹东118003

出  处:《辽东学院学报:自然科学版》2019年第1期13-17,共5页Journal of Liaodong University:Natural Sciences

基  金:国家自然科学基金项目(81602891);辽宁省教育厅科研项目(ldxy2017017);辽宁省高等学校创新人才支持计划项目(LR2016053);辽宁省大学生创新创业项目(2018117790057);辽东学院重点项目(2017ZD008).

摘  要:通过对染镧仔鼠皮质组织BAX mRNA和BAX蛋白表达的检测,探讨镧暴露对仔鼠皮质神经细胞BAX表达的影响。取Wistar雌性大鼠32只,随机分成对照组及低、中、高剂量LaCl3组,各LaCl3组饮水中镧(用LaCl3配制)浓度分别为0.25%、0.5%和1.0%,每组8只动物。实验动物以自由饮水的方式进行镧暴露。子代大鼠在断乳前经由母体血循环和吸吮母乳染镧,断乳后则通过自行饮水摄入。在整个实验过程中记录孕鼠及仔鼠体重,在仔鼠出生后49 d麻醉处死。取仔鼠皮质组织,应用实时定量PCR检测BAX mRNA、用Western-blot检测BAX蛋白表达水平变化。结果显示仔鼠皮质重量逐渐下降,高、中剂量LaCl3组仔鼠皮质重量低于对照组,皮质系数逐渐增加,差异有统计学意义(P<0.05),这提示仔鼠脑发育已经受损。仔鼠皮质组织BAX mRNA和BAX蛋白表达在各剂量LaCl3组之间出现差异,与对照组相比,高、中剂量LaCl3组仔鼠皮质组织中BAX mRNA和BAX蛋白表达量明显增高,差异有统计学意义(P<0.05);且随着LaCl3暴露剂量的增加,仔鼠皮质BAX mRNA和BAX蛋白表达水平逐渐上升,差异有统计学意义(P<0.05)。提示镧暴露导致的仔鼠大脑皮质发育损伤,可能与皮质BAX mRNA和BAX蛋白表达水平升高有关系。To investigate the effect of lanthanum exposure on the expression of BAX,BAX mRNA and BAX protein in the cortical nerve cells of offspring rats were measured.Thirty-two Wistar female rats were randomly divided into control group and low,medium and high dose LaCl3 groups.The concentration of lanthanum in water(prepared with LaCl3)was 0.25%,0.5%and 1.0%,respectively.Experimental animals were exposed to lanthanum by free drinking water.The offspring rats were exposed to lanthanum with maternal blood circulation and sucking breast milk before weaning,and then ingested by self-drinking water after weaning.The weights of pregnant and pups were recorded throughout the experiment.The offspring were anesthetized on the 49th day after birth and their cortex tissue were taken.The expression of BAX mRNA was detected by real-time quantitative PCR and the expression of BAX protein was detected by Western-blot.The results showed that the cortical weight of the rats was gradually decreased.The cortical weights of the high and medium dose LaCl3 group were lower than that of the control group,and the cortical coefficient was gradually increased,the difference was statistically significant(P<0.05),which indicated that the brain development of the offspring was impaired.The expression of BAX mRNA and BAX protein in the cortex of the rats were different among the groups.The expression of BAX mRNA and BAX protein in the cortical nerve cells of the high and medium dose LaCl3 group were significantly increased when compared with the control group,the differences were statistically significant(P<0.05).With the increase of exposure dose,the expression levels of BAX mRNA and BAX protein in the cortex of the rats were gradually increased,and the differences were statistically significant(P<0.05).It is suggested that the developmental damage of the cerebral cortex in the offspring induced by lanthanum may be related to the increased expression of BAX mRNA and BAX protein in the cortex.

关 键 词: 皮质 BAX 

分 类 号:R994.6[医药卫生—毒理学;医药卫生—药学]

 

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