虎源大肠杆菌ESBLs基因型的检测  

Detection of Extended-Spectrum β-lactamase Genotype and Antibiotic Resistance Analysis on Escherichia coli Isolated From Tiger

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作  者:牛鑫鑫[1] 冯涛[1] 何纪元 薛原[1] 曾祥伟[1] NIU Xinxin;FENG Tao;HE Jiyuan;XUE Yuan;ZENG Xiangwei(College of Wildlife Resources,Northeast Forest University,Harbin,150040,China)

机构地区:[1]东北林业大学野生动物资源学院,哈尔滨150040

出  处:《野生动物学报》2019年第1期70-74,共5页

基  金:国家自然科学基金(31502119);黑龙江省博士后科研启动金(LBH-Q14002).

摘  要:为检测虎源大肠杆菌的耐药性,根据超广谱β-内酰胺酶(ESBLs)基因型的流行情况指导临床合理使用抗菌药物,有效控制产ESBLs菌株的流行和感染。实验采用K-B法检测了30株虎源大肠杆菌对20种药物的耐药性,同时用PCR方法对确证的产ESBLs大肠杆菌进行TEM、CTX-M、OXA和SHV基因型检测。结果显示:21株虎源大肠杆菌为产ESBLs株,阳性率为70%。PCR扩增出了片段大小与预期相符的CTX-M型、OXA型、SHV型和TEM型基因。4个基因型的阳性率分别为50%、20%、6.67%、80%。可见,东北虎林园中产ESBLs的虎源大肠杆菌的检出率较高,TEM型为主要流行基因型。另外,发现产酶大肠杆菌比非产酶大肠杆菌的耐药性更高。This study tested the antibiotic resistance of Escherichia coli from tiger,developed guidance on the proper clinical use of antibiotics,and effectively controlled the infection of strains with extended-spectrumβ-lactamase(ESBL)genotypes.We tested drug resistance of 30 strains from tiger to 20 drugs by use of the KB method.E.coli with confirmed ESBLs genotype were tested for genotype of CTX-M,OXA,SHV and TEM by use of the PCR method.Twenty-one of the 30 strains were confirmed ESBL positive.The positive rate was 70%.Four different genotypes were detected among these ESBL isolates by PCR.The positive rates of genotypes CTX-M,OXA,SHV and TEM were 50%,20%,6.7%and 80%,respectively.Tiger-derived ESBL-positive strain showed high positive rates in Northeast Tiger Park,Harbin,and TEM was the most common genotype.ESBL-positive strains showed higher drug resistance than negative ones.

关 键 词:ESBLS  大肠杆菌 

分 类 号:S852.61[农业科学—基础兽医学;农业科学—兽医学;农业科学—畜牧兽医]

 

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