高效液相色谱法测定人血浆中阿托伐他汀的浓度  

Determination of atorvastatin concentration in human plasma by HPLC

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作  者:封永莉 欧亚青 边原[2] 张丽娟[2] 陈岷[2] 陈璐[2] 童荣生[2] FENG Yong-Li;OU Ya-qing;BIAN Yuan;ZHANG Li-juan;CHEN Min;CHEN Lu;TONG Rong-sheng(Personalized Drug Therapy Key Laboratory of Sichuan Province, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610072, China;Department of Pharmacy, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu 610072, China)

机构地区:[1]电子科技大学医学院,个体化药物治疗四川省重点实验室,四川成都610072 [2]四川省医学科学院·四川省人民医院药学部,四川成都610072

出  处:《实用医院临床杂志》2019年第1期113-115,共3页Practical Journal of Clinical Medicine

基  金:四川省卫生和计划生育委员会科研课题(编号:16PJ479);国家临床药学重点专科建设项目(编号:30305030698).

摘  要:目的建立高效液相色谱法(HPLC)测定人血浆中阿托伐他汀浓度。方法色谱柱:AglientEclipseHPLC色谱柱(150×4.6mm,5μm);流动相:乙腈:0.02mol/L醋酸铵缓冲溶液(冰醋酸调至PH=5.2)=40:60;流速:1ml/min。结果按上述色谱条件,阿托伐他汀保留时间为7.5min,专一性好,且基线无干扰。阿托伐他汀在0.41~14.45μg/ml范围内线性关系良好(R2=0.9993)。以0.64、3.03、12.89μg/ml为阿托伐他汀低、中、高浓度的血浆质控样品,日内和日间RSD均小于8%,相对提取回收率大于89%,且稳定性良好。结论该方法专一性强,简便快捷,适用于阿托伐他汀在特殊人群体内药代动力学及其相关性研究。Objective To establish a HPLC method to determine the concentration of atorvastatin in human plasma.Methods The chromatographic column used was Aglient Eclipse HPLC column(150×4.6 mm,5μm),mobile phase was 0.02mol/L acetonitrile-ammonium acetate buffer(40:60,pH5.2 adjusted with acetic acid)and flow rate was 1 ml/min.Results According to the chromatographic conditions described above,the retention time of atorvastatin was 7.5 min with high specificity and no baseline interference.The calibration curve of atorvastatin was linear in the range of 0.41 to 14.45μg/ml(R2=0.9993).When 0.6,3.03 and 12.89μg/ml were used as low,medium,and high concentration plasma quality control samples of atorvastatin,the values of intra-day and inter-day RSD were less than 8%and the relative extraction recovery were more than 89%with good stability.Conclusion The method is specific,simple and fast,and suitable for the pharmacokinetics and related research of atorvastatin in special population.

关 键 词:阿托伐他汀 HPLC 特殊人群 血药浓度 

分 类 号:R917[医药卫生—药物分析学;医药卫生—药学]

 

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