观赏海棠中McmiR160a的克隆及调控红叶着色的功能分析  

Cloning and functional assay of McmiR160regulatedred leaf coloration in Malus spp.

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作  者:罗容丽 李雨欣 张洁 张杰[1] 姚允聪[1] LUO Rongli;LI Yuxin;ZHANG Jie;ZHANG Jie;YAO Yuncong(Beijing Key Laboratory of New Technology in Agricultural Application/National Demonstration Center for Experimental Plant Production Education/Plant Science and Technology College,Beijing University of Agriculture,Beijing 102206,China)

机构地区:[1]农业应用新技术北京市重点实验室/植物生产国家级实验教学示范中心/北京农学院植物科学技术学院,北京102206

出  处:《北京农学院学报》2019年第1期14-19,共6页Journal of Beijing Agricultural College

基  金:长城学者项目(5045243001);北京农学院学位与研究生教育改革与发展项目(2018YJS037).

摘  要:【目的】为了验证观赏海棠叶片McmiR160a对花色素苷代谢的调控作用.【方法】以观赏海棠常色红叶品种'王族'为试验材料,确定miR160a的前体基因序列并对其靶基因进行预测,克隆McmiR160a前体序列并构建过表达载体并瞬时转入'王族'组培苗.通过高效液相色谱和qRT-PCR分析瞬时侵染植株中花色素苷的含量及其合成途径中结构基因的表达量.【结果】结果表明,在观赏海棠中过表达McmiR160a可以降低花色素苷的含量,同时降低其靶基因和花色素苷代谢途径关键基因表达水平.【结论】研究结果表明,观赏海棠中McmiR160a对花色素苷的合成具有负调控作用.【Objective】This experiment was to verify the regulation of miR160aon anthocyanin biosynthesis in Malus spp.leaves.【Methods】We used the leaves of ever-red leaf cultivar‘Royalty’of Malus spp.as experimental material.The sequence of miR160aprecursor gene and its target genes were predicted.The precursor sequence of McmiR160a was cloned and was transiently over-expressed into the‘Royalty’tissue culture seedlings.And then,the contents of anthocyanin and the expressions of key genes in the pathway of anthocyanin biosynthesis were analyzed by HPLC and qRT-PCR.【Results】The results showed that over-expressing of McmiR160ain Malus spp.leaves decreased the expression of its target gene,the anthocyanin contents,and the expressions of key genes in anthocyanin biosynthesis pathway.【Conclusion】The results suggested that McmiR160anegatively regulated anthocyanins biosynthesis Malus spp.

关 键 词:观赏海棠 花色素苷 McmiR160a 

分 类 号:S664.2[农业科学—果树学;农业科学—园艺学]

 

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