钙调蛋白对骨骼肌炎症的干扰效应  

The interference of Calmodulin on skeletal muscle inflammation

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作  者:史丹丹[1] 曹永英[2] 曹标[1] 刘幸卉[1] 术蓉[1] 谷瑞彩 肖将尉 丁茂超[1] 黄涛[1] 郭梦霞[1] 廖华[1] SHI Dandan, CAO Yongying, CAO Biao, LIU Xinghui, ZHU Rong, GU Ruicai, XIAO Jiangwei, DING Maochao, HUANG Tao, GUO Mengxia, LIAO Hua(1. Department of Anatomy, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering (Southern Medical University), Guangzhou 510515 China; 2. Sport College, Northwest University for Nationalities, Lanzhou 730030, China)

机构地区:[1]南方医科大学解剖学教研室广东省组织构建与检测重点实验室,广州510515 [2]西北民族大学体育学院,兰州730030

出  处:《免疫学杂志》2016年第9期756-761,共6页Immunological Journal

基  金:国家自然科学基金(81171724,81371924,81572102); 广东省科技计划(2012B031800146); 广东省自然科学基金自由申请项目(2014A030313276)

摘  要:目的探讨钙调蛋白(Calmodulin,CaM)对急性骨骼肌炎症反应的影响。方法心毒素(Cardiotoxin,CTX)肌内注射,诱导C57BL/6小鼠胫骨前肌(tibialis anterior,TA)急性损伤。肌损伤小鼠腹腔注射钙调蛋白抑制剂R24571或激动剂CALP1。分别检测TA肌组织自身抗原和TLRs表达以及单核/巨噬细胞的肌内浸润程度。结果 CTX注射导致急性肌损伤,TA肌内出现大量的炎性渗出,并伴随肌纤维坏死。损伤肌组织内CaM、自身抗原(Mi-2,HRS和Ku70)以及TLR3表达上调。较之CTX损伤组,CALP1处理导致损伤TA肌内单核/巨噬细胞数量增多,肌内自身抗原和TLR3 mRNA和蛋白水平上调,R24571则减少肌内炎性浸润并下调上述炎性介质。结论 CaM信号参与介导急性骨骼肌炎症反应。The Calcium/Calmodulin(Ca^2+/CaM) signaling has been reported to be essential for immune andinflammatory responses in peripheral tissues and organs. However, whether Ca M signaling interferes with muscleinflammation remains mostly unknown. In this study, we investigated the role of Ca M in acute skeletal muscleinflammation. B6 mice of 6-8-week old were induced acute myoinjury by Cardiotoxin(CTX) injection i.m., and thenintraperitoneally injected with Ca M inhibitor R24571 or CaM agonist CALP1, respectively. Muscle-autoantigens,TLRs, and mononuclear/macrophage cells infiltration were detected post-injury. We observed the increase levels ofCa M, muscle autoantigens(Mi-2, HRS and Ku70) and TLR3, as well as intensive intramuscular infiltration ofinflammatory cells after acute injury, while these indicators gradually reduced over time. We also found enhancedintramuscular infiltration of monocytes/macrophages, up-regulated m RNA and protein levels of muscle autoantigens(Mi-2, HRS and Ku70) and TLR3 in the damaged tibialis anterior muscle(TA) treated with CALP1, comparing tountreated one. In contrast, R24571 decreased the intramuscular infiltration, and down-regulated the m RNA andprotein levels of above inflammatory indicator. Thus, the present findings demonstrate Ca M-signal play a role inmediating acute muscle inflammatory responses.

关 键 词:钙调蛋白 肌损伤 炎症 自身抗原 

分 类 号:R685.4[医药卫生—骨科学;医药卫生—临床医学;医药卫生—外科学]

 

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